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Objective: Flow c ytometry fluorescence associated c ell sorting; FACS ; has been s uccessfully used to detect gene encoding at the protein level while fluor escent in situ hybridisation FISH ; is a valuable r esearc h tool in mol ecular cytogenetics and gene expression. We intended to eval uate the flow cytometric enumerati on of mRNA by FISH in intact living chondroc ytes. Methods: Designed mRNA s pecific oligodeoxynucl eoti de probes were s ynthesized and conj ugated with FITC. Human chondroc ytes were obtained from normal cartilage and cultured as phenotypicall y stable c ells in 1% alginate beads for one week. The chondroc ytes were then isolated from the alginate and were incubated with s pecific pr obes to hybridise the target mRNA molec ules. FACS detec ted fluoresc ence intensity of probes in the intact living chondroc ytes. The Mean Fluorescence Intensity MFI ; of the c ells due to the presence of the s pecific FITC-conjugated mRNA probe was then converted to numbers of mRNA mol ecules . Results: Single-stranded oligonucleotides pr obes shorter than 30 bas es freel y enter ed i ntact livi ng chondroc yte cytoplas m and annealed with the target mRNA. Uncombi ned probes and negati ve c ontrol probes were completel y washed away. The us e of number of supplementary probes linearly increased the fluorescent intensity of cells, but did not and gatifloxacin.
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Hon. Mr. Lingenfelter: -- Mr. Speaker, before orders of the day, I move, seconded by the member Cannington: That by leave of the Assembly, that notwithstanding rule 3 4 ; of the Rules and Procedures of the Legislative Assembly of Saskatchewan that when the Assembly adjourns on Thursday, April 20, 2000, it stands adjourned until Wednesday, April 26, at 1: 30 p.m. I move this, Mr. Speaker, in light of the fact that that is the Easter weekend so that families can prepare for a longer weekend than might otherwise be available to them. Motion agreed to. 1430 ; ORDERS OF THE DAY SPECIAL ORDER ADJOURNED DEBATES MOTION FOR COMMITTEE OF FINANCE BUDGET DEBATE ; The Assembly resumed the adjourned debate on the proposed motion by the Hon. Mr. Cline that the Assembly resolve itself into the Committee of Finance. Mr. Hermanson: -- Well, thank you, Mr. Speaker. As I was responding to the budget yesterday, somewhat in a state of shock, not believing what I was hearing from the Minister of Finance -- that he was actually going to increase taxes rather than cut taxes -- I thought have I got something wrong here? Did I miss something? But then I checked the papers and I checked with colleagues and people throughout Saskatchewan, and guess what? The entire province is sharing that same shock, that same dismay. We heard reports from the Saskatchewan School Trustees Association. They feel that this budget is an attack on children. We heard condemnation from SUMA Saskatchewan Urban Municipalities Association ; who see the expansion of the PST as a tax on municipalities. We heard from the Canadian Taxpayers Federation who were astounded that this government would raise taxes when other governments were lowering taxes. We heard from the roadbuilders' association who are astounded that this government does not recognize the deterioration of our roads and highways in Saskatchewan and are putting so little from this budget into highways. We heard from SAHO Saskatchewan Association of Health Organizations ; , from health care unions -- all these people were disappointed with the Finance minister's budget.
Krger C, University of Pretoria, Pretoria, South Africa Introduction: Previous studies of an association between dissociative experiences and EEG activity relied on measurements of a dissociative trait, or tendency to dissociate. This study measured spontaneous state symptoms of dissociation and concurrent EEG parameters. Methods: Sixteen psychiatric patients [mean age 32 19-53 ; years; M: F 3: 13] with a high tendency to dissociate [mean Dissociative Experiences Scale DES score 51 18 81 ; %] underwent an EEG recording. They completed the psychometrically validated, 56-item, self-report State Scale of Dissociation SSD ; immediately before and after a 15 minute EEG recording. The first 4 minutes of the EEG recording artifact-free ; were digitalised, Fast Fourier transformed, and spectrally analysed. The entire EEG recording was visually analysed. Results: Visual analysis of the EEGs showed 8 normal and 8 abnormal EEGs. The abnormalities varied between grades 1 and 3, and were located in the right temporal area in 5 of the 8 patients. Several SSD variables correlated directly with relative beta power, and indirectly with relative alpha, theta, and delta power, at central, temporal, and frontal electrodes. Regression analyses demonstrated that the depersonalisation and hypermnesia subscales of the SSD were the best predictors of the digital EEG variables. The administration of the SSD immediately after the EEG recording yielded the best predictions. Conclusions: The sample was small and medication exerted a confounding effect. Preliminary analyses suggest that SSD scores might predict the concurrent EEG parameters of dissociative states. The study population should be enlarged to include patients with a variety of psychiatric disorders and haldol.
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One year before presentation, the child and his 4-year-old brother were treated with a 6-week course of griseofulvin 12.5 mg kg per day for tinea capitis, and his mother was treated with topical antifungal agents for tinea corporis. Both the mother's and the brother's lesions were cleared with treatment, but our patient relapsed quickly after treatment and was given an additional 2-week course of griseofulvin and topical antifungal agents. After many months of topical antifungal therapy, the patient was evaluated at a pediatric dermatology clinic. Results of a KOH examination of his skin scrapings at that time were negative for fungal elements. A diagnosis of seborrhea petaloides was made, and topical tacrolimus was initiated. After one month of topical tacrolimus, the child presented to us with the lesions described. He was treated with griseofulvin 25 mg kg per day. After 2 weeks of treatment, the patient developed extensive scalp pustulation, which responded to a 5-day course of prednisone. Griseofulvin was continued, with full resolution of the dermatophytosis.
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