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Background: Interpretation of cellular and molecular pathogenesis of minimal traumatic brain injury is a clinical and scientific problem, especially due to the high prevalence of motor vehicle and other accidents. Pathogenetic brain mechanisms following traumatic impact are usually investigated by using models of severe or moderate trauma. Objective: Apoptotic neuronal degeneration after notable brain trauma is a well known phenomenon, but the source of its activation is not clear, especially after mild brain trauma. Method: We used a closed head weight-drop experimental model to induce minimal brain injury in mice. Pellets of 5, 10, 15, and 30 g were dropped on the right side of a mouse's head under light ether anesthesia. No abnormal behavioral or neurophysiological changes were seen following the head trauma. Morphological assessment was done 72 hours after the traumatic impact using TUNEL assay and silver staining. Results: We found gradual increase of TUNEL-positive and silver-impregnated cells number in different cortical and hippocampal regions of both injured and contralateral hemispheres. The threshold of traumatic impact that caused a significant activation was 10g to 15 g pellets evident by silver staining ; , and 15g to 20g for apoptosis. The most sensitive zones for trauma were anterior cingulate cortex and CA3 area of hippocampus. No bilateral hemispheric differences were found. Conclusions: Even closed head minimal traumatic brain injury can cause diffuse neuronal damage and apoptosis. This results correlate.
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G. B. Perunicic-Pekovic * 1, Z. R. Rasic2, S. I. Pljesa1, S. Stankovic3, M. Ilic3, D. Ristic4 Nephrology, University Hospital Zemun, Belgrade, Serbia, 2Endocrinology 3Biochemistry, Institute of Medical Biohemistry Clinical Centre of Serbia, 4Institute of Medical Research, Belgrade, Serbia Introduction: Major predictors of clinical outcome in dialysis patients are protein-energy malnutrition PEM ; and inflammation.A common mechanism for the development of CVD and malnutrition in dialysis patients may be cytokine activation. Methods: We examined 42 hemodialysis patients, recruited from our unit.The patients with diabetes, malignacy and acute infections were excluded from the study.We measured serum level of albumin, transferin, interleukin-6 IL-6 ; , tumor necrosis factor alpha TNF-alpha ; , high sensitivity C-reactive protein hs-CRP ; , and lipids. Body mass index BMI ; was recorded. Lean body mass LBM ; , fat mass and percentage body fat were measured by bioelectric impedance BIA ; . End diastolic pressure EDD ; , and ejection fraction EF ; were measured by echocardiography Results: There were 42 hemodialysis patients, aged between 24 and 68 years ing subjective global assesment SGA ; , 18, 5 % patients were well nourished, 24 55% ; patients were malnourished.There is negative correlation between CRP level and albumin r -0.31; p 0.05 ; and positive correlatin between CRP level and macroangiopathy r 0.33; p 0.05 ; . Results of the factor analysis have indicated six latent factors with 70, 5% of variance explained within all investigated parameters. The first factor F1 ; of all parameters has explained 19, 36% of variance.The highest factor loadings were found for plasma lipids. Significant factor loadings for the second factor F2 ; , with 15, 74% explained variance have the nutritional parameters.The third factor F3 ; , has two components, EF and IL-6. Conclusion: These data suggested that inflammatory markers in malnourished hemodialysis patients will identify patients at high risk of comorbidity and mortality.
In April, bills were introduced into both the U.S. House of Representatives and Senate by a bipartisan group of representatives and senators that would require the suspension of a number of antibiotics in animal feed. Many of these agricultural uses of antibiotics have been demonstrated to be an important source in the continued increase of antimicrobial resistance that is being encountered when treating human diseases. The bill is called "The Preservation of Antibiotics for Medical Treatment Act" or PAMTA. Those of us in infection control, infectious diseases or health care in general might want to consider contacting our elected officials concerning this bill if so inclined and suprax.
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Selective inactivation of the interleukin-10 gene in T cells results in enhanced contact hypersensitivity reactions A Roers, 2 L Ntambi, 2 E Strittmatter, 2 M Deckert, 1 W Stenzel, 1 CB Wilson, 4 T Krieg, 2 K Rajewsky3 and W Mueller5 1 Department of Dermatology, University of Cologne, Cologne, Germany, 2 Department of Neuropathology, University of Cologne, Cologne, Germany, 3 Department of Immunology, University of Washington, Seattle, WA, 4 Center for Blood Research, Harvard Medical School, Boston, MA and 5 Gesellschaft fur Biotechnologische Forschung, Braunschweig, Germany Interleukin-10 IL-10 ; is a potent suppressor of T helper 1 responses. It is secreted by activated macrophages, but also by the epithelia lining inner and outer body surfaces as well as by T and B lymphocytes. In order to elucidate the function of T cell derived IL-10, we have generated mice with selective deficiency for IL-10 only in T cells by means of the Cre lox-recombination system. Unexpectedly, the T cell specific IL-10 mutants spontaneously developed inflammatory bowel disease. The mutants also show enhanced contact hypersensitivity reactions as compared to control animals, while responses to nonspecific irritation of the skin are not enhanced. Furthermore, the T cell specific IL-10 mutants succumb to infection with an avirulent strain of T. gondii which is not harmful for wildtype animals. This phenotype is very similar to that of conventional IL-10 k.o. mice which lack IL-10 in all cell types and is probably a result of unbalanced T helper 1 responses. These results show that T cell derived IL-10 serves important regulatory functions which cannot be substituted for by IL-10 released from other cell types. Future experiments will aim at defining T cell subsets carrying the IL-10 dependent regulatory function.
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An increase in hCG levels that continued for at least 3 consecutive weeks. Relapse was diagnosed when serum hCG levels increased again 2 months after the cessation of chemotherapy. Patients who had drug resistance received other chemotherapy regimens. Statistical analysis was performed by using Kruskal-Wallis and Fisher exact tests to compare the medians and proportions, respectively. P .05 was considered statistically significant. Analysis was performed with SPSS 11.5 SPSS Inc, Chicago, IL.
Pain is one of the most prevalent symptoms experienced by people with cancer, affecting over 70% of patients, with up to 50% having less than acceptable pain control.2 Although opioids remain first-line agents, they are associated with a significant side effect burden that includes sedation, nausea and constipation. Recent evidence suggests that high-dose, long-term opioid therapy may have proneoplastic effects and the ability to predispose to infection through demonstrated immunosuppressive effects.37 Another promising attribute of the cannabinoids relates to their intrinsic analgesic properties. A significant body of preclinical evidence clearly demonstrates analgesia mediated through antinociceptive, antihyperalgesic, anti-allodynia and anti-inflammatory mechanisms Table 3 ; .12 Further, a number of preclinical studies have demonstrated synergistic sensory analgesia when opioids and cannabinoids are combined.38, 39 This is important because such combination therapy may permit prescribing opioids at lower doses, translating into reduced risk for opioid-related side effects. Sensory synergy has yet to be demonstrated in clinical trials although synergistic affective analgesia in humans has been demonstrated in an experimental thermal pain model.40 Data from a meta-analysis examining the use of cannabisderived treatments for neuropathic and MS pain support the use of cannabinoids in this setting.41 The cannabinoids significantly reduced pain p 0.03 ; compared with placebo. Findings from another study not included parallel those of the meta-analysis.14 Patients with chronic upper motor neuron syndrome due to traumatic spinal injury, ischemic infarction, intracerebral hemorrhage or MS ; in whom conventional therapy had not provided adequate pain relief experienced significantly decreased spasticity-related pain p 0.05 ; with the use of the cannabinoid nabilone.14 Data are emerging that indicate efficacy of cannabinoids in chronic pain as well.15 In one investigation in 30 patients with treatment-refractory chronic pain, nabilone was significantly more effective p 0.006 ; than placebo intent-to-treat analysis ; in reducing spinal pain intensity when added to standard analgesic therapy.15 Interestingly, these patients suffered from a wide range of ailments, including cervical syndrome, lower back pain, thoracic syndrome and others. GW Pharmaceuticals directed a European Phase III study in patients with cancer pain not responsive to opioids. Those receiving THC: CBD spray experienced significant pain reduction, measured by a numeric rating scale, compared to those receiving placebo p 0.014 ; .42 Further, 43% of subjects demonstrated more than 30% reduction in pain. Ongoing and planned studies may provide further evidence for the use of cannabinoids to manage pain. In addition to planned U.S. studies with nabilone in patients with chemotherapy-induced neuropathic pain, diabetic neuropathy and MS neuropathic pain, there are ongoing trials with THC: CBD in Europe and Canada in diabetic neuropathy and neuropathic pain characterized by allodynia. A U.S. Phase III clinical trial in patients with cancer pain is in the planning stage and cisapride.
Cephalosporins - 3rd Generation CEDAX 3 CEFIZOX 3 cefotaxime 1 cefpodoxime 1 ceftriaxone 1 FORTAZ 3 MAXIPIME 3 OMNICEF 3 ROCEPHIN 3 SPECTRACEF 3 SUPRAX 3 tazicef 1 tazidime 1 VANTIN 3 Clarithromycin BIAXIN BIAXIN XL clarithromycin CMV Agents CYTOVENE FOSCAVIR ganciclovir VALCYTE VISTIDE Cyclic Lipopeptides CUBICIN Dirithromycin DYNABAC Erythromycins e.e.s. ERYC ERYPED ERY-TAB erythrocin erythromycin erythromycin delayed release particles erythromycin ethylsuccinate PCE 46 3.
BMI body mass index; BPD-DG biliopancreatic diversion with distal gastrectomy; BPD-DS biliopancreatic diversion with duodenal switch; FU follow-up; IGB isolated gastric bypass; LAGB laparoscopic adjustable gastric banding; LASGB laparoscopic adjustable silicone gastric banding; LOS length of hospital stay; LVBG laparoscopic vertical-banded gastroplasty; NA not available; RNYGB Roux-en-Y gastric bypass. Reported at the beginning of the study. Complication rates varied in their definitions for reporting. Approximate, based on reported data. Represents 2 patients: 1 died 5 weeks postoperatively of a pulmonary embolism, and 1 died 1 year postoperatively of a myocardial infarction. Approximate median, 2 years; mean not available. #Does not include ventral hernias in 17% of patients.
By Lynn R. Webster, MD, FACPM, FASAM, Medical Director, Lifetree Pain Clinic, Medical Director and CEO, Lifetree Clinical Research, President, Utah Academy of Pain Medicine, Chief of Anesthesiology, Health South Salt Lake Surgical Center, Salt Lake City, Utah.
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Supattra Rungsimakan. Pharmacognostic properties of Khamin khruea. Bangkok : Chulalongkorn University, 2001. 188 p. T E16975 ; Weerachai Nanakorn. A study of the genus Terminalia Linn. Combretaceae ; in Thailand. Bangkok : Kasetsart University, 1981. 7 ; , 130 p. T.
To be positively linked to adenylate cyclase, however they show much lower affinity for 5HT, type antagonists Hoyer et a[., 1993 ; . Thus, the serotonin receptor is pharmacologically similar to 5HT2-typeand 5HT, recep tors, but is linked to the same second messenger system that 5HT, . It is possible that the receptor in the salivary glands may be similar to vertebrate 5HT2-type receptors, yet be linked to adenylate cyclase. Additional pharmacological data is needed to be better able to characterize the serotonin receptor in the salivary glands of Carausius. The pharmacological data supports the idea that serotonin is released from SN2, binds to receptors on the salivary gland cells, and elevates CAMP in the cells. There are many examples of.
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